Create running gel
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Material | 7.5% | 9% | 10% | 11% | 12.5% |
---|---|---|---|---|---|
30% Acrylamide | 2.5 | 3 | 3.3 | 3.6 | 4.2 |
1.5M Tris pH8.8 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
10%SDS | 100ul | 100ul | 100ul | 100ul | 100ul |
10%APS | 100ul | 100ul | 100ul | 100ul | 100ul |
TEMED | 10ul | 10ul | 10ul | 10ul | 10ul |
Water | 4.8 | 4.3 | 4 | 3.7 | 3.1 |