Corum:DNA Sequencing
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Overview
DNA Sequencing of miniprep DNA plasmid product at the University of Minnesota BioMedical Genomics Center.
Materials
- 5 μL miniprep plasmid DNA product
- 4 μL H2O
- 3 μL 2μM sequencing primer
Procedure
- Combine components into a 1.5 mL tube. Label tubes XYZ1, XYZ2, ..., where XYZ are your initials.
- Fill out and print the DNA sequencing form for individual tubes. Order details - Template: plasmid, Template mass: 500 ng, Template length: <length of plasmid>, Sequencing option: Sanger classic, Hardcopies: no hardcopies.
- Place the sample tubes and form into a plastic bag.
- Deposit at a BMGC sequencing drop off site.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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Contact
or instead, discuss this protocol.
Digital Signature
- SC 16:33, 25 June 2012 (EDT):