Cong T. Trinh:basic lab techniques

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Basic Lab Techniques

1) Safety is the priority. Rules are if you are not trained to do experiments that are potentially hazardous, consult your supervision first to discuss procedures in detail.

2) Learn how to use and maintain a lab notebook.

3) Learn the code of ethics of how to conduct research.

4) Learn to store chemicals in appropriate locations to avoid produce degradation.

5) Sterile techniques: preparing solid and liquid medium and solutions, working in the laminar hood, maintaining lab bench.

6) Pipetting

7) Solid and liquid medium preparation for cell cultures.

8) Perform cell growth and measure growth kinetics.

9) Perform culture transfer in solid and liquid media.

10) Genomic DNA extraction.

11) Plasmid mini-prep

12) Gel electrophoresis

13) Quantification of DNA concentration.

14) Prepare competent cells for chemical and electroporation transformation.

15) Transformation by chemical and electroporation methods.

16) Preparation of cell cultures for -80*C frozen stock

17) Preparation of anaerobic cultures.

18) Design primers to amplify a gene.

19) PCR technique

20) Perform DNA digestion and ligation for molecular cloning.

21) Understand and perform Gibson Assembly.

22) Basic Instruments: micro centrifuge, bench centrifuge, gel electrophoresis, UV imaging, shakers, incubators, HPLC, GC-MS, fermenters, balances, pH meters, UV spec, thermocycler.

23) Learn basic bioinformatics: design primers to amplify a gene, identify restriction sites for molecular cloning, design multiple genes in an operon, identify promoters, operators, start codons, stop codons, protein alignment (DNA 2.0)

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9Mar2012 MW