Cong T. Trinh:Ligation

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Ligation

Reaction components

Chemical	Volume (μL)
Plasmid	2.5   (Working concentration 5 ng)
Insert	        5
Buffer	        1
T4 Ligase	1
H2O	        0.5
Total	        10


Note:

Molar insert: plasmid ration = 0:1, 5:1, 10:1, 25:1
Room temperature ligation for 30 min
Inactivation: 65oC for 15 min



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