Cong T. Trinh:Ligation
From OpenWetWare
Jump to navigationJump to search
Ligation
Reaction components
Chemical Volume (μL) Plasmid 2.5 (Working concentration 5 ng) Insert 5 Buffer 1 T4 Ligase 1 H2O 0.5 Total 10
Note:
- Molar insert: plasmid ration = 0:1, 5:1, 10:1, 25:1
- Room temperature ligation for 30 min
- Inactivation: 65oC for 15 min