Combining Parts according to sequence homology
|Part number||Directionality/Efficiency||Lenght||Type||Location||Combine with|
|B0015||Bi,98% (F), 30->60% (R)||1||Double||In stock|
|B1001||Bi,90% (F), 75% (R)||1||Single|
|B0014||Bi,96% (F), 96% (R)||1||Double||In stock|
|B0011||Bi, ,95% (F), 86% (R)||46bp||Plate 1, 2A|
|pBR322 and its derivatives||pMB1||15-20|
|pACYC and its derivatives||p15A||10-12|
|pSC101 and its derivatives||pSC101||5|
|Incompatibility group||Negative control element||Comment|
|colE1[4, 5], pMB1||RNAI||controls processing of pre-RNAII into primer. pUC is derived from pBR322 (a single mutation in the pBR322 Primer RNA and deletion of the rop gene) which is derived from a pMB1 replicon, and cannot co-reside with the colE1 incompatibility group.|
|IncFII, pT181||RNA||controls synthesis of RepA protein|
|P1, F, R6K, pSC101, p15A||iterons||sequesters RepA protein|
Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.
Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.