Christian Niederauer/Notebook/RacingBacteria/Protocols/Glycerol Protocol

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Revive Bacteria from Glycerol Stock

  • Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Spreading_Bacteria streak the bacteria that adhere to the needle onto the surface of an LB agar plate containing the appropriate antibiotics. Incubate the plate overnight at 37°C
  • Scrape the frozen surface of the culture with a sterile inoculating needle or tip, and immediately immerse it in 2ml growth media within a 2059 snap-cap tube. Grow the bacteria overnight in a 37°C shaker.
  • Return the frozen culture to storage at -70°C

Source: doi:10.1101/pdb.prot4452