Chemical transformation buffer comparison
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Buffer | KCl | KOAc | CaCl2 | MnCl2 | RbCl | HACoCl3 | Glycerol | DMSO | Other | pH | Reference |
SF | 100mM | 10mM | 50mM | 10% | pH 6.1 | Hanahan patent | |||||
RFT | 30mM | 10mM | 50mM | 100mM | 15% | pH 5.8 | Hanahan patent | ||||
TFB-I | 30mM | 20mM | 50mM | 100mM | 15% | pH 5.8 | |||||
TFB-II | 75mM | 10mM |
15% |
10mM MOPS | pH 6.5 | ||||||
FSB | 100mM | 10mM | 45mM | 3mM | 10% w/v | pH 6.2 | Hanahan patent | ||||
FSB | 100mM | 10mM | 10mM | 44mM | ? | 3mM | 10% | 2x 3.5% | 5% Sucrose | pH 6.4->6.2 | Jessee patent |
FSB | 10mM | 10mM | 45mM | 3mM | 8% | 8% | pH 6.4->6.2 | Stratagene '525 | |||
CCMB80 | 10mM | 80mM | 20mM | 10% | 7% | 10mM MgCl2 | pH 6.4 | Invitrogen '464 | |||
TB | 250mM | 15mM | 55mM | 7% | 10mM PIPES pH 6.7 | pH 5.7->6.2 | Inoue90 |