# Centrifuge, Superspeed (Avanti)

## BECKMAN AVANTI CENTRIFUGE (B414 & B464)

CAPTAINS: Ganesh Pusapati - B435 & Hernan Espinosa - B439

BEFORE USE:

1. New user MUST be trained by the captain or present users

2. ALWAYS sign and add info in logbook, especially if an issue occurs.

3. Biochemistry Department students and staff have priority when using this and all centrifuges. Outside users MUST have prior approval from Marc Perez in B483 and are responsible for bringing their own rotors, tubes, etc.

INSTRUCTIONS FOR USE:

1. You must be fluent in the principals and use of centrifuges before using this instrument. See your RA (first) or the equipment captain (second) if you are not.

2. Short instructions on how to use these centrifuges are on the centrifuge cover.

3. These machines will run Beckman or Fiberlite brand rotors, the fiberlite ones are lighter and quicker to use.

REMINDERS:

1. Check (and clean if necessary) the rotor and centrifuge before and after each run.

2. Always use a balance to balance your tubes, never "eyeball" the tubes.

3. Always assume the cap may leak and underfill the tube. To determine the fail-safe level hold the tube at the complementary angle (complement of the angle it is in the rotor) and fill until water flows out of the tube. This is your max. fill volume.

4. Remain with the 'fuge until it comes up to speed.

5. When removing your samples check to see if they leaked. If so clean the rotor and centrifuge. If an O-ring broke replace it and clean the centrifuge.

Please report all issues to Marc Perez, B483

## BECKMAN ROTOR USE

 HOW TO USE: 1. Beckman brand rotors will work in any Beckman centrifuge. Select the proper rotor: 2. Make sure the rotor doesn't have any liquid or other residue in it before you load it. 3. Check that both O-rings are intact, replace if necessary. 4. Balance your tubes with an electronic balance and do not overfill, assume the cap might leak and fill to the proper height with this in mind. 5. Load tubes in rotor, balance the laod. 6. Attaching the lid to rotor also attaches the rotor to the centrifuge spindle. Lock the lid and rotor down the one knob, check to see that the lid is tight. 7. Complete your run as appropriate. 8. When removing your samples check to see if they leaked. If so clean the rotor and centrifuge. If an O-ring broke replace it and clean the centrifuge.  ADDITIONAL INFORMATION: Extra O-rings are located in the clear drawers over the centrifuges. Beckman SpinKote (lubricant) is located in the drawers over the centrifuges. Use a small dab on spindle/cover lockdown threads to allow easy installation and removal of covers/rotors. Vacuum grease: Use a small dab on new or "dry" O-rings. The vacuum timer and rotor speed must be at zero before the door can be opened.  Beckman Coulter: 1-800-742-2345 Rotor/Tubes Manual: File:JSeries.pdf Quick Reference: https://ehs.stanford.edu/reference/centrifuge-safety Please report all issues to Marc Perez, B483 Marc Perez | March 2023