Carrico:Recipes
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Carrico Lab at Stony Brook
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2XYT Media (to make 1L)
- Combine: 900mL distilled water, 16g Bacto Tryptone, 10g Bacto Yeast Extract, 5g NaCl
- pH to 7.0
- Add water up to 1L
- Autoclave
DNA Gel Electrophoresis
50x TAE Buffer (to make 1L)
- 242g 2M Tris base
- 57.1mL 1M glacial acetic acid
- 200mL 100mM EDTA
- Add water up to 1L
Note: To dilute 50x to 1x, add 20mL of 50x to 980mL water
Protein
10x Laemmli Running Buffer (Tris glycine)
- 30.3g Tris base
- 144g glycine
- 10g SDS
- Add water to 1L
Coomassie Brilliant Blue (CBB)
- Dissolve 0.25g CBB in 90mL methanol:water (1:1 v/v) and 10mL glacial acetic acid
- Filter through Whatman #1 filter paper to remove particulate matter
- Stain gel for at least 4 hours in 5 volumes of CBB
Protein Gel Destain Solution
- 125mL methanol
- 35mL glacial acetic acid
- Add water to 500mL
- Store at room temperature
- Destain for up to 24 hours