Calendar:ImperialWetLabNotebook2009iGEM/2009-9-1
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- James Field 14:05, 1 September 2009 (EDT):
AM
- SLIC Primer design
- PCV FMA assay
PM
- Transforming biobricks into cells & plating
- Miniprep prep for the three Edinburgh cellulases
- Dineka Khurmi 04:30, 2 September 2009 (EDT):
AM
- Restriction digest on I13504, B0034 (1), B0034 (2), K118011 (1), K118011 (2), R0011, K098995
- Gel extraction on GFP (I13504) from last week (Placed in freezer labelled GRP GE 1/09/09)
PM
- Run K118011 (2) on a 1.5% gel and cut out gel (give to Jim)
- Dephosphorylation for dirty ligations for RBS (B0034) + AK3
- Ligation for RBS (B0034) + AK3 (placed in float in water bath in cold room)
