CRISPR in Nannochloropsis

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CRISPR/Cas9 techniques in Nannochloropsis

Production and screening of Cas9

Production and screening of sgRNA

An effective sgRNA should not have terminal modifications and needs to present in the nucleus. In Nannochloropsis sgRNAs have been produced using protein coding promoters and exogenously produced and transformed into the cells. To generate more efficient sgRNAs from RNA polymerase II promoters, self-cleaving ribozymes can be appended to the sgRNA gene.

Screening for mutations

Published studies

Future directions