Biomod/2014/NTU/Material

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<html> <head> <title></title> <link href="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/css/bootstrap.css&action=raw&ctype=text/css" rel='stylesheet' type='text/css' /> <link href="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/css/animate.css&action=raw&ctype=text/css" rel="stylesheet" type="text/css" media="all"> <link href="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/css/style.css&action=raw&ctype=text/css" rel='stylesheet' type='text/css' /> <script type="text/javascript" src="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/js/jquery.min.js&action=raw&ctype=text/js"></script> <script type="text/javascript" src="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/js/slidejs&action=raw&ctype=text/js"></script> <script src="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/js/slideknowledge&action=raw&ctype=text/js"></script> <script> $(function () { $("style[media*='screen']").remove(); $("link[href*='favicon']").remove(); //fix heading var h1 = $(".firstHeading").text().split("/"); $(".firstHeading").text(h1[h1.length-1]); $("tr:odd").addClass("odd"); }); function slideload(){ $('#fadein').fadeIn(6000); } </script> <style>

.body {

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 min-width: 0 ;
 padding-top: 55px;
 margin-left: auto;
 margin-right: auto;

}

  1. column-content #content {
   padding: 0em;
   margin: 0;

}

  1. content{
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}

  1. contentSub, #search-controls, .firstHeading, #footer-box, #catlinks, #p-logo, #toctitle ,#top-section ,#column-one ,#footer, #siteSub,#jump-to-nav,.printfooter,.visualClear

{

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.start:hover{ background-color: #8B29A4; transition: background-color 0.5s linear; } </style> <link href="http://openwetware.org/index.php?title=Biomod/2014/NTU/Templates/css/slidecss&action=raw&ctype=text/css" rel='stylesheet' type='text/css' /> </head> <body onload="slideload()" style="background-color: #C9BCCC;">

<img src="http://openwetware.org/images/e/ef/Supplement.JPG">

Supplement

Material

start

Materials

Scaffold:Tilibit, Single-stranded scaffold DNA, type p7249, 0.5 ml at 100 nM
Staples (More than 50 bases):Genomics Biosci & Tech
Staples (Less than 50 bases):NTU Biotech center
Purification:Amicon Ultra-0.5 mL Centrifugal Filters for DNA and Protein Purification and Concentration
Virus:Professor Chwan-Chuen King lab, College of Public Health, National Taiwan University

<img src="http://openwetware.org/images/1/1b/Material.png"/>

The H5N2 virus had been treated with Beta propyl lactone for 3 days, which ensures that the virus is deactivated.