Biomod/2012/UTokyo/UT-Komaba/Experiment
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Experiment
Please click on the section you want to learn more about.
Dynamic System
<html> <a href="http://openwetware.org/wiki/Biomod/2012/UTokyo/UT-Komaba/Experiment/Normal_Bistable"><img src="http://openwetware.org/images/3/31/Biomod_2012_UTokyo_UT-Komaba_Experiment_Icon_NormalBistable.png" width="250px" height="110px" alt="Normal Bistable" /></a> <a href="http://openwetware.org/wiki/Biomod/2012/UTokyo/UT-Komaba/Experiment/Indirect_Bistable"><img src="http://openwetware.org/images/7/79/Biomod_2012_UTokyo_UT-Komaba_Experiment_Icon_IndirectBistable.png" width="250px" height="110px" alt="Indirect Bistable" /></a> <a href="http://openwetware.org/wiki/Biomod/2012/UTokyo/UT-Komaba/Experiment/Trioscillate_System"><img src="http://openwetware.org/images/8/88/Biomod_2012_UTokyo_UT-Komaba_Experiment_Icon_TrioscillatorSystem.png" width="250px" height="110px" alt="Trioscillate System" /></a> </html>
DNA Origami
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DNA Tablet
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Lab Notes
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Glossary
Buffers
| TAE | Essential buffer for making origami, which contains TAE |
|---|---|
| Mg2+ | Essential for keeping the shape of origami |
| Smix | Buffer contains a lot of essential chemicals |
| DTT | Buffer which contains DTT |
| dTTP | Buffer which contains dTTP |
| BSA | Buffer which contains BSA |
| mQ | Pure water |
Enzymes
| NBI | Buffer which contains DNA nickase |
|---|---|
| Bst | Buffer which contains DNA polymerase |
| tt-RecJ | Buffer which contains DNA exonuclease |
DNAs
| M13 | Scaffold strand |
|---|---|
| VII | Strand for Dynamic system |
| XII | Strand for Dynamic system |
| DII | Strand for Dynamic system |
| NII | Strand for Dynamic system |
| inhV | Inhibitor to VII-autocatalisys |
| inhX | Inhibitor to XII-autocatalisys |
| inhQ | Inhibitor to QII-autocatalisys |
Templates
| CvII | Autocatalysis template for VII |
|---|---|
| CxII | Autocatalysis template for XII |
| V to inhX | Template which produces inhibitor to XII-autocatalisys from VII |
| V to inhQ | Template which produces inhibitor to QII-autocatalisys from VII |
| X to inhV | Template which produces inhibitor to VII-autocatalisys from XII |
| X to inhQ | Template which produces inhibitor to QII-autocatalisys from XII |
| Q to inhV | Template which produces inhibitor to VII-autocatalisys from QII |
| Q to inhX | Template which produces inhibitor to XII-autocatalisys from QII |
| D to V | Template which produces VII from DII |
| N to X | Template which produces XII from NII |
