Berglund:Spec and Qubit calculators
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Spec:
DNA (ng/ μL) = Absorbance260nm * dilution * 50
RNA (ng/µl) = Absorbance260nm * dilution * 40
Calc number of molecules:
(Absorbance260nm * dilution * 0.05 * 6.02x10^17)/ (MW= 320.5*#nucleotides)= Molecules/μL
Calculation for Qubit:
? (ng/mL) * 100 * (1/1000 mL) * (1 g/10^9 ng) * (10^6 μL/1 L) *
(1 mol/32370.5 g) * (10^6 μmol/1 mol) = ? μM
? μM * (1 L/10^6 μL) * (1 mol/10^6 μmol) * 6.02x10^23 = moles/ μL
moles/ μL * μL sample = # molecules
