Quick sheet for use of radioactivity EDITED BY AEM JULY 2008
Arrival of the radioactivity
1 Put on your dosimeters and gloves and lab coat
2 Place the P32 in the locked refridgerator
3 Hang up the accompanying sheet on the wall by the locked door. Write either the letter "A" for ATP for the letter "C" for CTP on the sheet.
PUT ON YOUR LAB COAT AND GLOVES BEFORE ANYTHING ELSE
1 Check the areas that you plan to use with a geiger counter.
2 Prepare radioactive pipette box for tip disposal
3 lay out equipment so that it is easy to get at without knocking things over
4 put shield up and pipette men/tips/waste container behind it as well as other equipment you need
5 have kimwipes and water bottle close at hand
6 have extra gloves ready, never walk around with your gloves on when handling radioactivity it is better to just change gloves (throw away in radioactive container regardless if you suspect they are contaminated or not)
7 Have ice bucket nearby, all other chemicals that need thawing
8 If using PCR machine, have turned on ect.
USING the RADIOACTIVITY
1 Turn on the geiger counter and point it towards you so that as you work you can check yourself
2 Take radioactivity out of fridge. Be careful not to contaminate area when pipetting. Use Filter tips when working with the stock solution.
3 RECORD AMT USED ON THE SHEET HANGING ON THE WALL BY THE LOCKED DOOR
4 clean up after yourself as you go, then you are less likely to contaminate yourself
5 When you are finished with the radioactivity, place back in fridge
6 dispose of all solid waste in the proper shielded can
7 Dispose of all liquid waste in the shielded flask with the funnel
8 Check the floor if you carried gels/gel rigs across the floor
9 If you are storing radiolabelled PCR product, RNA, ect, you must store it in a shielded container that is labelled with your name and date
10 Don't store radiolabelled gels
11 after you are done with your experiment, you must use the geiger counter 1cm from any object and slowly check the area where you have been for contamination. This includes:
your lab coat
goggles if used
the ice bucket
12 Clean up your spill/contamination and if it is bad you need to tell Amy or Andy.
Be careful and go slow
1. Fill out the documentation of radiation
2. Check your area before and after you use it for contamination
3. Label all of your tubes with initials and date and store in a shielded area