Berglund:PCR Buffer
From OpenWetWare
Jump to navigationJump to search
10x PCR Buffer (LabTaq)
- 6.25ml 4M KCl (fc=500mM)
- 5ml 1M Tris pH 8.9 (fc=100mM)
- 0.5ml Triton-x-100 (fc=1%)
- 0.75ml 1M MgCl2 (fc=15mM)
- 37.5ml water
6x DNA dye (for splicing gels)
- 100µl 1M Tris pH7.5 (fc=1.7mM)
- 4ml 100% glycerol (fc=6.7%)
- 2.4ml 0.5M EDTA pH 8 (fc=20mM)
- 12mg bromophenol blue (fc=0.3mM)
- 15mg xylene cyanol (fc=0.5mM)
- water to 10ml
Sybr Green dye
- 1.5ml 6x DNA dye
- 1.2µl 10,000 SYBRI dye