BXMU-PROJECT

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<div id="main_saurs" style="width:960px;"> <div class="content_list"> <a href="#result" style="color:black;background:#fff294;font-size:28px;font-family:Tekton Pro;">Result</a> <a href="#methods" style="color:black;background:#99CCFF;font-size:28px;font-family:Tekton Pro;">Method</a> </div> <div style="padding:0; margin:5px 0; height:1px; overflow:hidden; font:1px/1px simsun; border-bottom:1px dashed #999"></div> <a id="result"><h2><b>Result</b></h2> <div style="padding:0; margin:5px 0; height:1px; overflow:hidden; font:1px/1px simsun; border-bottom:1px dashed #999"></div> </a>


<h3>1.Construction of BL21(DE3)pET28a-bis-His tag GDH-NOX</h3> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/7/7f/Ixmu-tem2.png"> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/0/02/Ixmu-tem3.png"> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/e/ed/Ixmu-tem4.png"> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/7/75/Ixmu-tem1.png">


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<h3>2. Expression and purification of bis-His tag GDH-NOX</h3> <p>Our final results are as following table:</p>

<img style="margin:0 auto;" src="http://openwetware.org/images/9/9b/Xmu_table1-1.jpg">
<h3>3. Assembly of enzyme nanoring mediated by nickel ion</h3>

<p>xxxxxxxxxxxxxx</p>

<h4>4. Tanning experiment</h4> <p></p> <h5>4.1 DHA synthesis by purified enzyme</h5> <h5>4.2 DHA synthesis by crude enzymee</h5> <h5>4.3 Sunless Tanning</h5>


<div style="padding:0; margin:5px 0; height:1px; overflow:hidden; font:1px/1px simsun; border-bottom:1px dashed #999"></div> <a id="methods"><h2><b>Methods</b></h2> <div style="padding:0; margin:5px 0; height:1px; overflow:hidden; font:1px/1px simsun; border-bottom:1px dashed #999"></div> <h3>1 Genetic Manipulation</h3>

<p>The method used to form the GUVs is “Vesicle Formation from emulsion”. This method consists in a controlled hydration of dried or nearly dried films of lipids deposited on a solid surface, in this case electrodes. </p>

<h3>2. Gene expression and enzyme purification</h3> <p>xxxxxx</p>

<h3>3. Nickel ion mediated enzyme assembly</h3> <p>xxxxxx</p>

<h3>4. In situ synthesis of DHA and sunless tanning </h3> <p>xxxxxx</p>

<h3>5. Characterization and analytic approaches</h3> <p>xxxxxx</p>

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