BXMU-PROJECT
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<a href="#result" style="color:black;background:#fff294;font-size:28px;font-family:Tekton Pro;">Result</a> <a href="#methods" style="color:black;background:#99CCFF;font-size:28px;font-family:Tekton Pro;">Method</a>
Result
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1.Construction of BL21(DE3)pET28a-bis-His tag GDH-NOX
<img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/7/7f/Ixmu-tem2.png"> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/0/02/Ixmu-tem3.png"> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/e/ed/Ixmu-tem4.png"> <img style="float:center;margin:0px 20px 0px 0px;border:2px solid black;" src="http://openwetware.org/images/7/75/Ixmu-tem1.png">
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2. Expression and purification of bis-His tag GDH-NOX
Our final results are as following table:
<img style="margin:0 auto;" src="http://openwetware.org/images/9/9b/Xmu_table1-1.jpg">
3. Assembly of enzyme nanoring mediated by nickel ion
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4. Tanning experiment
4.1 DHA synthesis by purified enzyme
4.2 DHA synthesis by crude enzymee
4.3 Sunless Tanning
Methods
1 Genetic Manipulation
The method used to form the GUVs is “Vesicle Formation from emulsion”. This method consists in a controlled hydration of dried or nearly dried films of lipids deposited on a solid surface, in this case electrodes.
2. Gene expression and enzyme purification
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3. Nickel ion mediated enzyme assembly
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4. In situ synthesis of DHA and sunless tanning
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5. Characterization and analytic approaches
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