OUR TEAM
LAB 3 WRITE-UP
Original System: PCR Results
PCR Test Results
Sample Name |
Ave. INTDEN* |
Calculated μg/mL |
Conclusion (pos/neg)
|
Positive Control |
--- |
--- |
N/A
|
Negative Control |
--- |
--- |
N/A
|
Tube Label:___ Patient ID: ____ rep 1 |
--- |
--- |
---
|
Tube Label:___ Patient ID: ____ rep 2 |
--- |
--- |
---
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Tube Label:___ Patient ID: ____ rep 3 |
--- |
--- |
---
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Tube Label:___ Patient ID: ____ rep 1 |
--- |
--- |
---
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Tube Label:___ Patient ID: ____ rep 2 |
--- |
--- |
---
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Tube Label:___ Patient ID: ____ rep 3 |
--- |
--- |
---
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* Ave. INTDEN = Average of ImageJ integrated density values from three Fluorimeter images
Bayesian Statistics
These following conditional statistics are based upon all of the DNA detection system results that were obtained in the PCR lab for 20 hypothetical patients who were diagnosed as either having cancer or not having cancer.
Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)
Calculation 1: The probability that the sample actually has the cancer DNA sequence, given a positive diagnostic signal.
- A = [text description] = [frequency shown as a fraction] = [final numerical value]
- B = [text description] = [frequency shown as a fraction] = [final numerical value]
- P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
- P(A|B) = [answer]
Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.
- A = [text description] = [frequency shown as a fraction] = [final numerical value]
- B = [text description] = [frequency shown as a fraction] = [final numerical value]
- P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
- P(A|B) = [answer]
New System: Design Strategy
We concluded that a good system Must Have:
- [Must have #1 - why? short, ~4 or 5 sentences]
- [Must have #2 - why? short, ~4 or 5 sentences]
We concluded that we would Want a good system to have:
- [Want #1 - why? short, ~4 or 5 sentences]
- [Want #2 - why? short, ~4 or 5 sentences]
We concluded that a good system Must Not Have:
- [Must Not Have #1 - why? short, ~4 or 5 sentences]
- [Must Not Have #2 - why? short, ~4 or 5 sentences]
We concluded that a good system Should Avoid:
- [Should Avoid #1 - why? short, ~4 or 5 sentences]
- [Should Avoid #2 - why? short, ~4 or 5 sentences]
New System: Machine/ Device Engineering
SYSTEM DESIGN
KEY FEATURES
We chose to include these new features
- Feature 1 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
- Feature 2 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
- Etc.
[OR]
We chose keep the devices the same as the original system
- Feature 1 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
- Feature 2 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
- Etc.
INSTRUCTIONS
New System: Protocols
DESIGN
We chose to include these new approaches/ features
- Feature 1 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
- Feature 2 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
- Etc.
[OR]
We chose keep the protocols the same as the original system
- Feature 1 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
- Feature 2 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
- Etc.
MATERIALS
PROTOCOLS
- Step 1
- Step 2
- Etc.
- DNA Measurement and Analysis Protocol
- Step 1
- Step 2
- Etc.
New System: Research and Development
BACKGROUND
DESIGN
Primers for PCR
Our primers address the following design needs
- Design specification 1 - explanation of how an aspect of the primers addresses any of the specifications in the "New System: Design Strategy" section
- Design specification 2 - explanation of how an aspect of the primers addresses any of the specifications in the "New System: Design Strategy" section
- Etc.
New System: Software
[THIS SECTION IS OPTIONAL. If your team has creative ideas for new software, and new software is a key component included in your new protocols, R&D, or machine design, you may describe it here. You will not receive bonus points, but a solid effort may raise your overall page layout points. If you decide not to propose new software, please delete this entire section, including the ==New System: Software== header.]
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