BME100 s2016:Group2 W1030AM L1

BME 100 Spring 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Logan Luke Earl Brown Erik Drager Bailey Gasvoda Ishitha Jagadish Destinee Martin-Karim LAB 1 WRITE-UP Independent and Dependent Variables Independent Variable: Amount of the inflammation inducing agent (LPS: lipopolysaccharide), because the amount of the dosage will be changed. Dependent Variable: The amount of inflammatory protein (Inflammotin) that is present in the blood of the elderly, because this is what is being measured for results. Also, the amount of Inflammotin is dependent on the amount of dosage of LPS. Experimental Design Groups 6 groups total (10 people per group): Group 1: 10 mg given Group 2: 8 mg given Group 3: 6 mg given Group 4: 4 mg given Group 5: 2 mg given Group 6: Control group (0mg) We start the dosage at 10 mg and gradually lower the dosage for each group to test the effects on the protein levels in order to find the lowest dosage possible that will still be potent. Age 65 years old (age of retirement) Number of subjects per group 10 subjects per group Subject Selection Randomization: Put each patient's name and gender in a program that will randomly select 10 subjects (5 male, 5 female) who will have applied to the study (advertisements asking for volunteers for the study will be put out) and place them into each group. Bias: To eliminate bias, we will use a computer system to select the groups. Control: The control group will be given 0 mg to compare with the the groups which have been given a dosage. Including: age of 65, healthy Excluding: people with the pre-existing/persisting medical condition of inflammatory problems Sources of Error and Bias Error could arise during the subject selection process. Subjects could have other factors affecting their drug absorption capabilities. This error could be accounted for by manipulating the analysis of the data according to how well the drug was absorbed by the study's participants. Subjects are selected randomly using a computer program; this could result in any combination of subjects in a particular group. To correct this error, experimenters could hire computer science specialists to make a good quality program which will hopefully randomize groups well. The patients' diet, weight, blood pressure, and any outstanding health problems or medications can affect how the lipopolysaccharide works in the body. During the study, patients are fed a controlled diet and encouraged to exercise regularly to minimize the sources of error involving health (diet, weight, blood pressure, other outstanding health problems or medications). The study will be completed with a double-blind procedure. This means that the test subjects and the person administering the drugs to each test subject do not know which treatments are placebos and not the actual lipopolysaccharide.