BME100 f2018:Group8 T1030 L6
|BME 100 Fall 2018|| Home |
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Wiki Editing Help
LAB 6 WRITE-UP
Overview of the Original Diagnosis System
There were 17 teams of 4-5 students each, and we diagnosed 34 patients total. For each patient, we had three replicates. In order to further prevent error, we used positive and negative controls, in order to compare them to our patients. Our ImageJ calibration controls were used to ensure that ImageJ was working properly and would provide accurate results for our patients. Using three images per patient allowed us to ensure that the ImageJ images were accurate. There were a few inconclusive test results from the experiment; however, the inconclusive test results did not affect the conclusion of the PCR test. As a result of the inconclusive test results, there were inconclusive diagnoses for the patients. There was blank data for Group 1, which may have skewed the test results. We also encountered problems when micropipetting the soultions into the vials. This may have caused a mistake in our data and therefore cause a mistake in the class data.
Intro to Computer-Aided Design
Feature 1: Consumables
Since we did not make changes to the PCR machine itself and only changed the set up of the lightbox, standard sized consumables may still be used without issue. We will utilize the same consumables as the iteration of the PCR machine and process that we worked with in previous labs. Our consumable parts are interchangeable with those used in standard PCR experiments- standard micropipettors, liquid reagents, PCR tubes and tube racks, and so on. Therefore, our product does not include specific "very important" consumable parts that are only applicable to our kit; our product consists of our original lightbox design, a fluorimeter, and and our magnetized stand.
Feature 2: Hardware - PCR Machine & Fluorimeter
The OpenPCR machine did not go any changes in our design and will still be used in the same manner. We changed the design of the light box in order to make the fluorimeter setup less difficult; the fluorimeter will simply be placed into an elevated platform inside our light box, which has a sliding lid and a peephole in order to more completely block out light. We will use other standard processes and equipment in order to perform the remaining steps of the PCR reaction.