BME100 f2017:Group14 W1030 L3

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OUR TEAM

Name: Lydia Gabric
Name: Anshul Krishnan
Name: Christopher Ward
Name: Sara Bacon
Name: Charmayne Seaton

Hypotheisis

Urine-Buffer solution infected with Chlamydia trachomatis bacteria when applied to an immunoassay strip will display parallel lines.

Variables

Independant Variable: Urine Buffer Solution

Dependant Variable: Immunoassay strip

Materials

- 2; 3 mL pipettes

- 10 mL 0.5 N Sodium Hydroxide buffer

- 10 mL Tris-HCL pH 9.0 with 0.02% sodium azide buffer

- Immunoassay Strip apparatus (strip present in plastic casing)

- Urine infected with Chlamydia trachomatis bacteria

- Uninfected Urine

- Random Urine samples (unknown infection status)

Gold Standard

Our Gold Standard will be the Chlamydia tests performed at labs

Precursor & Procedure

Precursor (will test each one by the gold standard and our product):

- Phase 1: Gather 100 samples of infected urine from patients, in clinics, showing high probability of having Chlamydia.

- Phase 2: Gather 100 samples of healthy urine from patients, in clinics, that show no signs of Chlamydia

- Phase 3: Gather 100 samples of unknown urine from random patients, in clinics, that are unaware if they have it or not.


Procedure: - Gather listed materials, assemble apparatus and ensure safety equipment is in place

- Mix the buffer solutions with the Urine samples at hand.

- With the mixture from the previous step, we will place a sample of that solution onto the immunoassay strip apparatus.

- Wait for results.

-Compare results with the Lab results.


Data Collection & Processing

Raw Data: Testing hypothesized experiment against Lab STD results

Processing Data: Apply Chi-Squared test, to search for correlation between Lab results and Hypothesized experiment results


Statistical Analysis

From our statistical analysis, we can conclude that the spree band accurately calculate temperature, but not accurately calculate heart rate. We came to this conclusion because the t-test value for temperature was 1.0910^-21 which is less than .05 which means there is a significant difference. While the t-test value for heart rate was .41 which is greater than .05 which means there is not a significant difference. If the data shows a significant difference it means that the data did not happen by chance. The temperature and heart rate r^2 values were .03 and .4 which means that the data has a very low correlation which proves that there is a weak relationship between the two variables.



Temperature

BME100L3Temp.png Temperature Gold Standard vs Spree

BME100TempVsTrials.png Temperature Vs. Trials

Screen Shot 2017-09-25 at 6.06.50 AM.png

Heart Rate

Heartrategoldstandardvsspree.png

Goldstandardspreeheartrate.png

Screen Shot 2017-09-26 at 7.05.57 PM.png