BME100 f2015:Group9 8amL1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable is the dose of an inflammation inducing agent lipopolysaccharide. The different levels for this variable will be 10mg, 8mg, 6mg, 4mg, 2mg, and 0mg as the control group. The lipopolysaccharide is the independent variable based on the fact that this is the variable that needs to be manipulated to get results that answer the real world problem.
The dependent variable will be the amount of the inflammatory protein measured in the blood samples, because the amount of inflammatory protein can be influenced by the amount of lipopolysaccharide. This will be measured by the enzyme-linked immunosorbent assay (ELISA).
Subjects can be found through random selection in area agencies on aging. An equal number of men and women will be selected for each age group defined in the group selection above. This will be done by assigning the subjects a number from 1 to 30. The assigning of numbers will be done within each category of subjects. For example, males between the ages 56 and 60 will be numbered 1-30 as will the females between the ages of 56 and 60. A random number table will be used to put an equal number of subjects from each category into the six groups. Factors such as preexisting disease will be factored out of the selection pool as these must be accounted into the experiment based on the fact that these factors would affect the data in the experiment.
Sources of Error and Bias
Ideally there would be one person for every age between 56 and 65 in each group tested, but there is no guarantee that will be the case. The drug could affect males and females differently so there will be equal amounts of each for all the groups. Varying diets, sleep schedule and daily habits such as smoking, drinking and exercise could contribute to different results. Reactions and side effects to the medication could cause error in the results.