BME100 f2015:Group6 8amL5

BME 100 Fall 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Error creating thumbnail: File missing Name: Zach Humphreys Error creating thumbnail: File missing Name: Kianna Browning Error creating thumbnail: File missing Name: Cameron McAllister Error creating thumbnail: File missing Name: Blake Browning Error creating thumbnail: File missing Name: Colten Hedrick Error creating thumbnail: File missing Name: Anuj Agrawal LAB 5 WRITE-UP PCR Reaction Report We used the pipettor to transfer the PCR reaction mix, patients 1 and 2's replicate mix, the positive DNa/primer mix, and the negative control. The key to this was proper technique. When drawing fluids, only push the pipettor to the first stop, but when ejecting fluids, push all the way to the second stop. Failure to do either of these will result in a difference in the actual amount as compared to the desired amount of substance. Next, we placed the tubes with the substances into the OpenPCR device. Fluorimeter Procedure Smart Phone Camera Settings Type of Smartphone: iPhone 6S Flash: Off ISO setting: Auto White Balance: Auto Exposure: Auto Saturation: Auto Contrast: Auto Camera set-up Distance between the smart phone cradle and drop = Placing Samples onto the Fluorimeter Set volume on pipettor to 80 microliters. Place slide into fluorimeter such that the holes that will be used are aligned with the blue LED light. Firmly attach tip to pipettor. Pipette 80 microliters of SYBR Green I onto one of the middle holes of the slide. Eject pipette tip into waste basket. Firmly attach another tip. Pipette 80 microliters of .25 concentration of the calf thymus into the hole right above the SYBR Green I. Use timer on camera to take pictures of the solution in the dark under the blue LED light. Dispose of the liquid on the slide into the liquid waste basket. Repeat steps for the .5, 1, 2, and 5 concentrations of the calf thymus. Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved PCR Results: Summary Our positive control PCR result was 3986962.3 μg/mL Our negative control PCR result was 2182675.3 μg/mL Observed results Patient 78778: Patient 63691: Conclusions Patient 78778: positive because values above positive control Patient 63691: positive because values above positive control