BME100 f2015:Group6 1030amL6
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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LAB 6 WRITE-UP
Overview of the Original Diagnosis System
In order to properly analyze the disease-associated SNP 17 teams of 6 students were each given 2 of the 34 patients to analyze. Each group performed OpenPCR on the samples to determine if the disease-associated SNP was present. In order to minimize error each group was given three samples for each patient to analyze. Also, positive and negative controls were provided to ensure that all groups were basing their data on the same values for a positive and negative conclusion. For the fluorimeter tests three images were taken for each droplet which used the same amount of SYBR Green and sample of patient solution. When analyzing the ImageJ the same circle was used to analyze all the droplets which allowed for less variation to ensure more accurate data. The data for 30 patients was succesfull in finding a conclusion. However only 16 of the diagnoses were accurate in analyzing the presence, or lack thereof, of the disease associated SNP. The class produced only two inconclusive results and two patients did not receive data. The class data table was included to make it easier to see the data being discussed. Some problems that were encountered were that it was difficult to take accurate photos of the droplets because the camera would take a burst of pictures which had a flash which could have affected the SYBR Green. Also, the camera was at different lengths from the droplet which slightly influenced the ImageJ which would have changed the data, causing some of the conclusions be inaccurate.
What Bayes Statistics Imply about This Diagnostic Approach
Intro to Computer-Aided Design
Feature 1: Consumables
The materials are shipped in a cold container to prevent degradation or reaction within the solutions. The SYBR Green will be in darkened tubes to limit the amount of light that enters the tube which could degrade SYBR Green. The micropipetter tips will be sealed in a container to prevent any possible contamination. The PCR tubes and the glass slides will each be kept in separate sealed containers to avoid possible contaminants. Upon arrival the disease primers, SYBR Green and buffer controls should be placed in a lab refrigrator taking extra precautions to keep SYBR Green covered to prevent degradation. Our design improves on the previous one because it allows for all the consumables needed to be available at the same time as well as prevent possible contamination by keeping the tips, slides, and tubes sealed.
Feature 2: Hardware - PCR Machine & Fluorimeter
The changes we made were both to the PCR machine. A battery pack was added (shown in red) such that the PCR machine is now self-sufficient on power. In addition, the PCR machine was made smaller yet still holds 16 PCR tubes. These changes collectively make the PCR machine smaller, more portable, and cheaper. Making it easier for researchers who need this technology on the field and making it a viable option not only for those who can not afford an advanced one, but a viable option for those who do, but need a portable version for there research.