BME100 f2015:Group5 8amL1

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Owwnotebook icon.png BME 100 Fall 2015 Home
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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Name: Tyler Mitchell
Name: Austin James
Name: Maryam Aosuwailem
Name: Sultan Alanazi
Name: Logan Martin
Name: Amber Sogge


Independent and Dependent Variables

Independent Variables: Amount of lipopolysaccharide in mg. The amount of lipopolysaccharide is the independent variable because it is the variable that is controlled and changed to measure the amount of inflammotin it produces.

Dependent Variable: Amount of imflammotin produced. The amount of imflammotin is the dependent variable because it is the variable that is measured in elderly people as a result of the change in the amount of lipopolysaccharide.

Experimental Design

2. 100 patients will be grouped according to prescribed dosages, with 20 patients in each of the five groups. The prescribed dosages for this experiment are 0mg, 3mg, 5mg, 7mg, and 10mg. The patients receiving 0mg will serve as the control group. This experiment will be double blind, meaning that the test subjects will not know what dose is prescribed to them. Blood samples will then be taken to analyze the level of inflammation in each recipient and in each group. This analysis will determine the lowest effective dose for this drug.

                      Table 1.1

Dosage |||||| Year ||||||||| Years | Years | Years | Years

Group |||||| Range ||||||||| 60-64 | 65-69 | 70-74 | 75-79

0mg group ||||| 60-80 |||||| 5 ||||||||| 5 ||||||||| 5 ||||||||| 5

3mg group ||||| 60-80 |||||| 5 ||||||||| 5 ||||||||| 5 ||||||||| 5

5mg group ||||| 60-80 |||||| 5 ||||||||| 5 ||||||||| 5 ||||||||| 5

7mg group ||||| 60-80 |||||| 5 ||||||||| 5 ||||||||| 5 ||||||||| 5

10mg group |||| 60-80 |||||| 5 ||||||||| 5 ||||||||| 5 ||||||||| 5

Subject Selection

3. To select our subjects, we will begin by making sure that the patients do not have any allergic reactions to the medication. Test subjects within the age group of 60 to 79 years old will be randomly selected for this experiment. Each subject will be placed within their respective age groups (specified in Table 1.1) and then will be randomly selected into a dosage group. All recipients will take one dose of lipopolysaccharide and then the protein will be measured in the blood sample using ELISA. Each patient must fast for 9 hours before getting a blood sample. The blood samples will then be analyzed for the corresponding effectiveness of each dose.

Sources of Error and Bias

One source of bias could be ignoring the data from those that have only slight reaction or the experimenters limiting the information given to only include data that supports the hypothesis. The experimenters could fix this problem by providing all of the data gathered and clearly explaining outliers. Another source of error would be if the elderly people develop a condition that is unrelated to the study but requires them to leave the study or alters their results. This problem could be solved by recording the data but recording it as an outlier and explain the circumstances surrounding it. Also, if the individuals react in different amounts of time to the medication the complete effect of the medication could be missed in the measurement if only a few measurements are taken. For example the highest concentration of inflammotion may take longer to appear in some and appear faster in others. To fix this problem, the experimenters would take a blood sample every three hours for nine hours after the first dose and record all of the data.