BME100 f2015:Group5 1030amL5

BME 100 Fall 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Taylor Barda Name: Doug Brown Name: Mary Cauley Name: Steven Stamm Name: Alexandra Davis Name: Andrew Soich LAB 5 WRITE-UP PCR Reaction Report The team experienced no trouble with pipetting the samples for Lab C. Although there were various skill levels in the group regarding micro-pipetting prior to Lab C, the pre-lab reading assisted in helping those that had not previously worked with micropipettes. During the lab and all proceeding labs, we thought it best to utilize two micropipettors: one experienced and one using a micropipette for the first time. With this setup, we always had one knowledgeable member paired and prepared to assist the less experienced member while they improve their skills. The final reactions did end up having the exact same amount of liquid due to the spot on precision of the micro-pipettes. There was some liquid left in the tubes containing DNA samples, however the PCR reaction mix was used entirely by the end of the lab. Our labeling scheme did not have to be changed after the initial labeling. Fluorimeter Procedure Smart Phone Camera Settings Type of Smartphone: iPhone 6 Flash: Off ISO setting: 2000 White Balance: Auto White Balance Exposure: 1/15 sec Saturation: N/A Contrast: N/A *Note: All setting specifics obtained through image metadata via OpenWetWare. Camera set-up Distance between the smart phone cradle and drop = 11.00 cm Placing Samples onto the Fluorimeter Slide glass slide into designated grooves on the fluorimeter After obtaining the necessary quantity of dye solution in the micropipette, start by placing a single drop in the middle circular groove of the first row on the glass slide. Continue by placing another drop in the next groove back. Place a third drop in-between the two preexisting drops and continue dispensing the dye in the pipette until there is a globular collection of dye in the middle of the slide. Repeat Step #2 with the necessary quantity of DNA solution. Center the DNA/Dye solution bubble so that it is cut in half by the light of the fluorimeter. Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA "High Concentration (5micrograms/mL sample):" "Low Concentration (.5micrograms/mL sample):" "No Concentration (0micrograms/mL sample):" Calibrator Mean Values Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved PCR Results: Summary Our positive control PCR result was 26.73 μg/mL Our negative control PCR result was -4.76 μg/mL Observed results Patient 51283 : The images for Patient 51283 were most similar to the images taken of the negative control. -57.01 μg/mL Patient 60999 : The images for Patient 60999 were most similar to the images taken of the positive control. 23.23 μg/mL Conclusions Patient 51283 : This patient is negative for the tested SNP as the PCR Product Concentration is nearest to that of the negative control. Patient 60999 : This patient is positive for the tested SNP as the PCR Product Concentration is nearest to that of the positive control.