BME100 f2015:Group4 8amL1

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Kendra Starkel
Name: Austen Bennett
Name: Drew Higgins
Name: Michael Vietz
Name: Bhavna Ramesh
Name: Anastasia Wyatt

LAB 1 WRITE-UP

Independent and Dependent Variables

Independent Variable: Dosage of lipopolysaccharide (mg) - levels of lipopolysaccharide(LPS) will be changed throughout the trial and the effect of these changes will be recorded.
Dependent Variable: Levels of the inflammatory protein Inflammotin - the levels of inflammatory protein depend on the dosage of LPS.

Experimental Design

Groups
The trial will contain 6 groups of 10 including men and women of various ages. At least one subject from each age group is preferred. There will be one control group who will be given a placebo and five experimental groups who will be given a different dosage of the drug (0mg, 6mg, 8mg, 10mg, 12mg, 14mg).
This will allow for an adequate sample size that gives an accurate representation of the elderly population while being conscious of costs these groups were allotted.


Age
Between the ages of 70-85 years old in order to accurately represent a major sector of the elderly while being able to include a variety from the selected age group.


Number of subjects per group
10 subjects per group.


Procedure
Each group of subjects will be given their selected dosage of the lipopolysaccharide once a day for a period of three days at 9:00am. Subjects will be instructed to take the drug with a meal that will be provided. Subjects' blood protein levels will then be measured with the ELISA test 30 minutes, 1 hour, and 2 hours after ingestion of the drug.




Subject Selection

Doctors will be informed about the trial and various patients will be referred by their personal doctors. Patients will join the trial voluntarily. Along with doctor referrals, a survey will be conducted at various nursing homes and senior centers to recruit prospective participants.
These two different methods of selection were implemented in order to ensure randomization of the sample size.
Exclusions: Patients who have major health complications will be excluded from the trial along with patients currently prescribed with an anti-inflammatory medication.






Sources of Error and Bias

Several variables could affect the outcome of the trial including: prior medical conditions, presence of current medications, current health status, allergies, race, and diet.
In order to account for these possible errors, participants will be required to fill out a medical status questionnaire. Subjects will not be informed of the dosage they are receiving.