BME100 f2015:Group4 1030amL6
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LAB 6 WRITE-UP
Overview of the Original Diagnosis System
In this procedure, thirty-four patients were tested for a disease-associated SNP with seventeen groups analyzed the DNA of two patients each. Each group contained six students. Several procedures were followed to inhibit the acquisition of error within the results. First of all, three samples were taken from each patient to ensure quality results and a lack of contamination affecting them. Secondly, one sample for both a positive and negative control were taken for comparison purposes in the analysis portion of the laboratory procedure. Furthermore, ImageJ was calibrated to eliminate error in the measuring of area and RAWINTDEN of the drops. Lastly, each sample and control had three drop images taken to hinder error in the fluorescence portion of the experiment. For the thirty-four patients, only two patients yielded inconclusive results. Additionally, for two patients there was blank data entered. For the other patients, thirteen got a positive conclusion and seventeen received a negative conclusion. The only problem encountered in the proceeding of this experiment was slightly blurred photos for a good amount of the samples. This led to inaccurate results on the ImageJ measurements.
What Bayes Statistics Imply about This Diagnostic Approach
One possible source of error is the shaking of the camera during the fluorescence activity. This would blur the light in the picture, therefore increasing the measured area of the drop. A second source is the contamination of the PCR samples during the first half of the laboratory experiment, which could distort the samples to either positive, negative or inconclusive results. Finally, a third source of experimental error is the contamination of the positive and negative samples. When comparing the samples to these values, the group may come to the wrong conclusion due to an inaccurate positive and negative value for the controls.
Intro to Computer-Aided Design
Feature 1: Consumables
Seeing as our NRDS device appears and works like the Open PCR machine, the same tube sizes will work within our device. The consumables included in our device include pipette tips, a pipettor hanger, PCR mix tubes (empty), PCR mix tubes (including PCR mix), and DNA sample tubes. We consider these components to be the most important in the PCR process.
The NRDS Consumables include:
Feature 2: Hardware - PCR Machine & Fluorimeter
The Open PCR machine and fluorimeter will be integrated into our system like they were in the lab. After amplifying the DNA in the PCR machine, the fluorimeter will be used to identify subjects testing positive and negative for diseases, when compared to positive and negative controls. The only change to this system will be nesting the lid of the PCR machine in the device itself and creating a new phone clip for androids and iPhones that allows for adjustment when using the fluorimeter.
Two issues arose in the progression of the PCR and fluorimeter labs. The first was present in closing the lid to the PCR machine, and the second consisted of propping an android phone up in the fluorimeter step. Therefore, our group chose to change these two aspects: the lid will now be integrated into the PCR machine so that it does not project outwards, and a stronger and more adjustable clip will be included with the fluorimeter material.