BME100 f2015:Group1 8amL1
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LAB 1 WRITE-UP
Independent and Dependent Variables
Objective/Purpose: The purpose of this lab is to determine the lowest possible dose of an inflammation inducing agent (lipopolysaccharide) that will increase a newly discovered inflammatory protein (Inflammotin) in the elderly.
Independent: The independent variable of the experiment is the dosage of the inflammation inducing agent (lipopolysaccharide) given to the subject. Measured in milligrams. Subjects will be given doses of 5mg, 6mg, 7mg, 8 mg, and 9mg.
Dependent: The dependent variable of the experiment is the inflammatory protein (Inflammotin) levels of the subjects after the inducing agent is delivered. Measured in blood samples using ELISA.
Subjects will be chosen based on average health expectancies for that age group. So people with chronic heart disease or any other type of serious health defect that might affect experiment results will be rejected from the test group so that the test results will be relevant to the average person in that age group.
Sources of Error and Bias
Error: Groups may not be large enough to have variance due to budget issues. Age range may be too large. Previous health issues that could affect how the drug affects them. Subject could possibly be taking other medications. The lowest dose being given is 5 mg when it is possible that a lower dose might work, however, because the lab is running on limited funds, they are not being tested at this time.