BME100 f2015:Group14 8amL1

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Owwnotebook icon.png BME 100 Fall 2015 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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Our Team

Name: Jared Johns
Name: Ryan Bartholomew
Name: Morgan Cobban
Name: Cara Beauvais
Name: Zachary Smith
Name: Jon Talos

Lab 1 Write-up

Independent and Dependent Variables

We will be testing the effects of LPS (Lipopolysaccharide) on the levels of new inflammotin in the elderly.
Independent Variable: Dosage of LPS (Lipopolysaccharide)

  • These are the values we will be controlling in the experiment

Dependent Variable: Inflammotin protein levels

  • Protein levels are dependent on the level of LPS administered and will be measured

Experimental Design

Groups
11 Groups
1-10mg, and Placebo with 0mg
Group 1: 1mg
Group 2: 2mg
Group 3: 3mg
.
.
.
Group 10: 10mg (Established minimum effective concentration)
Group 11: 0mg (Control)

Age
"Elderly", defined as anyone who is 65 years of age or older.(65+)
There is no limit for our age range in order to recruit more subjects. A health screening will be conducted on each patient to ensure that they do not have any medical history that may affect the results. With this screening, if the potential subjects meet the standard of age and relative health they will be deemed applicable for our experiment.

Number of subjects per group
Each group will consist of 10 people (in blocks of 5 male, 5 female) per group. This sample is meant to represent the population.

Drug Administration
The lipopolysaccharide is to be administered on a daily basis. Because of this schedule, the inflammation of the subjects will also be recorded daily.

Subject Selection

This will be a voluntary experiment that will be promoted in nursing-homes nationwide. Once we have a number of volunteers we will randomly assign 5 males and 5 females into each group. Each patient will be screened for pre-existing health conditions; therefore, our standard is not only age, but also wellness. Patients will be monitored by nurses to assure their medicine is being taken consistently, and at the same time each day.



Sources of Error and Bias

  • People in nursing homes could be more dependent on care.

Fix: Assign nurse to administer drug on regular schedule.

  • Contraction of disease during trial.

Fix: Maintain controlled, sanitary environment to reduce risk of disease.

  • Unknown factors (previous health conditions not detected during initial screening).

Fix: Ensure initial screening is extensive.

  • Dietary factors.

Fix: Restrict and monitor diet

  • Activity levels (Past and present).

Fix: Screen for exceedingly active/inactive subjects.