BME100 f2015:Group11 1030amL6
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LAB 6 WRITE-UP
Overview of the Original Diagnosis System
BME 100 took two patients DNA samples and made three replicas of each DNA and then had a positive and negative control. Each sample was placed into a tube and primer gets added to each one. The samples are then placed into the OpenPCR machine which was pre-set to certain cycles. These cycles start with the heated lid which is 100*C, then the initial step of 95*C for 2 minutes, then 25 cycles of denature at 95*C for 30 seconds, anneal at 57*C for 30 seconds, and extend at 72*C for 30 seconds, then the final step at 72*C for 2 minutes, and final hold at 4*C. The samples were left in the OpenPCR machine and were removed once the cycles were completed. Next we took each of the samples and looked at them with the fluorimeter box which emitted a light through the sample droplet and revealed a green fluorescence which showed that the disease was present in our patients. Once all of the data was gathered there were a few groups that received inconclusive results, most groups received successful results, and only one group received blank data. There were many errors that could have occurred in this experiment that could have altered the final results of whether the patient intact does have the disease or not. Some of these could have been not putting the correct amounts of each sample in the tubes and also incorrectly placing the primer incorrectly. It was assumed that all of these things were done correctly but there could always be an error.
Intro to Computer-Aided Design
With TinkerCAD there are templates available online that you can download and change or you can create your own. With this lab the design template for the open PCR system was previously downloaded and it was able to be changed and altered to create a new system that was custom designed. When compared to solid works it is easier to create your own pieces rather than with TinkerCAD where the pieces are remade but you have to download each individual piece. It is also more in depth on solid works so you have the ability to be more creative and change more aspects of your design.
Feature 1: Consumables
Very important consumables are supplies specific to the PCR kit that must be included with the new design of the PCR machine. Our new design does not require any new consumables because the only changes to the design are a light to show the phase of the PCR reaction and an increased amount of tube holding holes. Therefore, we would include the same basic consumables in our kit.
List of Consumables
Feature 2: Hardware - PCR Machine & Fluorimeter
We addressed two weaknesses in the PCR machine to make it more effective. We added more holes for the tubes to be placed in so the machine can run more tests at once and more data can be gathered. We also added a light to the front that will change colors to indicate what stage of the cycle that the machine is on. These improvements will allow more tests to be done at once and will allow the tester to know what stage the cycles are on so they can know how much time is left and when the temperatures are changing.