BME100 f2015:Group10 1030amL5

BME 100 Fall 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM JOEL HARNISCH GERRIT ORTHLIEB BRANDON BURGERS SAI ADONI SERGIO MEJIA LAB 5 WRITE-UP PCR Reaction Report Our team conducted the procedure of pipetting the way it was described in the pre-lab readings and watching video modules helped with getting familiar on how to use a pipette. Each team member understood the difference between the first and second stop on the pipettor. The first stop on the pipettor is used to collect a sample once finger has been removed from stop and sample has been collected. The second stop is used to release all of the sample from the pipettor into the desired location. The final reactions had the same amount of liquids for all tubes because the pipettor was calibrated to intake a volume of 120 micro-liters. Sample of DNA and PCR reaction mix were left over because the procedure was focused on taking a specific amount of sample for PCR reaction. Prior labeling of tubes from previous investigation worked efficiently for labeling tubes and avoiding cross contamination for this investigation. Pipetting was done successfully in the group by following the pre-lab reading steps and instructions on day of the lab. Fluorimeter Procedure Brandon: Smart Phone Camera Settings Type of Smartphone: iPhone 6 Flash: No flash ISO setting: 800 White Balance: Auto Exposure: 100% Saturation: 100% Contrast: 0% Camera set-up The smartphone must be set camera and placed in a cradle at distance such that the image will not be blurred and the camera can focus. The camera must be perpendicular to the drop on the slide. The distance between the cradle and fluorimeter was about 7 centimeters. At this distance, we could capture a clear, accurate image of the samples in the fluorimeter. Distance between the smart phone cradle and drop = 7 IF THIS FOLLOWING PORTION IS BLANK PLEASE GIVE BRANDON A "0%" FOR CONTRIBUTION Placing Samples onto the Fluorimeter [Step One: A 160 microliter drop of water should be placed in between the first two rows of slides and the drop must be clear and round.] [] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Collection and Analysis The images for the high, low, and zero Calf Thymus DNA were deleted and misplaced. Calibrator Mean Values Calibration curves Positive PCR Control Negative PCR Control PCR Results: PCR concentrations solved PCR Results: Summary 527991 = 2653.632x+2542.374, x=198.011 Our positive control PCR result was 198.011 μg/mL Our negative control PCR result was 55.592 μg/mL Observed results Patient 27800: 1-1: 177.01 1-2: 204.93 1-3: 174.42 Mean: 185.45 Patient 35993: 2-1: 41.308 2-2: 42.067 2-3: 55.749 Mean: 45.04 Conclusions Patient 27800: Patient 27800 has a mean concentration of 185.45 ug/mL, a mere 8 ug/mL away from the positive control, indicating that the patient contains the mutation indicated by the PCR test. Patient 35993: Patient 35993 has a mean concentration of 45.04, below the threshold for the negative control, indicating that the patient does not contain the mutation indicated by the PCR test.