BM20
From OpenWetWare
Jump to navigationJump to search
BM20 media
- 10% low endotoxin FCS
- 20% L-cell supernatant
- 5% horse serum
- 1% glutamine
- 1% sodium pyruvate
- no Abx
- to final volume with low endo DMEM
1L
- 630ml low endotoxin DMEM
- 100ml low endotoxin FCS
- 200ml L-cell supernatant
- 50ml horse serum
- 10ml glutamine
- 10ml sodium pyruvate
- No Abx
