BISC220/S10: Mod 1 Media

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β-galactosidase Series Media Recipes

Solution Composition
Luria Broth 1% Tryptone
0.5% Yeast Extract
1% NaCl
2% agar (only in plates)
Ampicillin Stock = 25 mg/ml in water
IPTG (Isopropyl β-D-1-thiogalactopyranoside) Stock 1 mg/ml
Na2CO3 Stock 1M
Z-buffer 60 mM Na2HPO4
60 mM NaH2PO4
1 mM MgSO4
10 mM HCl
0.27% β-mercaptoethanol
ONPG (ortho-Nitrophenyl-β-galactoside) Stock 4 mg/ml
Laemmli Solution (2x Sample Buffer) 0.125 M Trisma Base
4% SDS
20% glycerol
10% β-mercaptoethanol
Gel Running Buffer 0.92 M Glycine
0.05 M Trisma Base
0.1% SDS
Coomassie Blue Stain 0.1% Coomassie Blue
50% Methanol
10% Acetic Acid
Destain 40% Methanol
7% Acetic Acid