BISC220/S10: Media Recipes

From OpenWetWare
Jump to navigationJump to search
Wellesley College     BISC 220     Cellular Physiology

BISC220 S10 template image.png

Home        Lecture Syllabus        Lab Calendars/Point Distribution        Assignments        Resources        Glossary       
Enzymes        Secretory Pathway        Cytoskeleton        OWW Basics              

Cytoskeleton Series Media Recipes

Solution Composition
PBS (Phosphate Buffered Saline) 1X 2.68 mM KCl
1.46 mM KH2PO4
136.9 NaCl
8.1 mM Na2HPO4
PBST (PBS with Tween 20) 1X PBS with 0.25% Tween 20
Trypsin Stock = 0.25%
PEM Buffer 1 M Pipes
0.05 M EGTA
0.02 M MgCl2 6H2O
Cell Culture Media 1 Packet DMEM Powder
0.15% NaHCO3
10% Newborn Calf Serum
Trypan Blue 0.1% Trypan Blue in water