BISC220/S10: Media Recipes
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Cytoskeleton Series Media Recipes
| Solution | Composition |
|---|---|
| PBS (Phosphate Buffered Saline) 1X | 2.68 mM KCl 1.46 mM KH2PO4 136.9 NaCl 8.1 mM Na2HPO4 |
| PBST (PBS with Tween 20) | 1X PBS with 0.25% Tween 20 |
| Trypsin | Stock = 0.25% |
| PEM Buffer | 1 M Pipes 0.05 M EGTA 0.02 M MgCl2 6H2O |
| Cell Culture Media | 1 Packet DMEM Powder 0.15% NaHCO3 10% Newborn Calf Serum |
| Trypan Blue | 0.1% Trypan Blue in water |
