BISC220/S10: Hitachi Spec Instructions

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Wellesley College     BISC 220     Cellular Physiology

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Hitachi Spectrophotometer Operation

Press ENTER (2X)
Under Data Mode, select 1 for absorbance, ENTER
Test Set Up, ENTER
Sample num., select 1, ENTER
Wavelength, set desired wavelength, ENTER
Delay, set 1 or 2 seconds, ENTER
FORWARD KEY (wait for proper wavelength to appear on screen)
Place blank material in the reference and test positions, AUTO ZERO KEY
Remove blank material from test position and replace with sample, START KEY
Repeat for remaining samples, PAPER FEED KEY, take printed results
NOTE: Not all specs will print your results - be sure to record data in your notebook!