BISC219/F11: Assignment Series2 Outline Summary

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Assignment Series 2 Outline & Summary

Please construct a complete yet concise statement of the overall topic of investigation in Series 2 and the experimental question & goals of this classical (forward) genetics approach to understanding the structural and functional relationship between a gene and the product it encodes.

Construct and outline the entire experimental approach to achieving the our experimental goals in Series 2, including all of the following parts to our project: test for a true breeding mutant, linkage analysis to locate the gene associated with the mutation to a chromosome (any of the 6), mapping the mutation's location on a chromosome, complementation analysis to see if the mutation has been previously characterized, defining the gene sequence change responsible for the gene product change that is associated with the functional defect in the mutant through DNA sequence analysis and comparison with wild type gene sequence to find functionally significant sequences in a gene that encode important parts of the gene product, using bioinformatics (Wormbase or BLAST OR other public search engines) to find out if the proposed structurally/funcationally significant regions of the gene or gene product in C. elegans have homologs/orthologs in other species to broaden the context and significance of our findings. This outline can be done in outline form or as a flow-diagram.

Please use this outline to compose a summary in prose of how you will determine the gene and the exact location of the gene responsible for a particular mutant phenotype, how you will find the exact sequence change that is likely to be responsible for the abberrant phenotype, and if that gene or gene product change could be significant in other species. Note that this summary should NOT be a detailed list of exactly what you DO in lab in chronological order, but should, instead, be a brief summary of each part of the work that is included in our project. Imagine yourself trying to explain your project to someone who knows nothing about C. elegans genetics or about these mutant phenotypes.

Grading Rubric

Assignment Lab 2– 10 points

At or Above Standard Below Standard Possible


& Experimental Question
Constructed a clear, concise, and accurate description of the overall topic, the experimental question(s) and goals of the Series 2 investigation Topic or experimental question(s)/goals missing, inaccurate, partially accurate; contains too much tangential information or is too simplified 2 __/2
of Experimental Approach in Series 2: Classical or Forward Genetics
Constructed a complete, clear, concise, and accurate outline or flow diagram of ALL parts of Series2 including: test for true breeding, linkage testing to determine which chromosome the gene responsible for the mutation is found, mapping the mutation on a chromosome, complementation testing to determine if new mutation or previously characterized gene and mutation, DNA sequencing analysis & bioinformatics work to find structurally significant parts of the gene and functionally significant parts of the gene product, and to broaden the context and significance of the findings but a search for find homologs/orthologs. Outline/diagram is missing elements, is inaccurate or partially inaccurate; contains too much tangential information or is too simplified. 4 __/4
Narrative summary of project
Constructed a clear, concise, accurate, and logically developed description of the experimental approach to locating and characterizing the gene and gene mutation responsible for a phenotypic alteration from wild type Experimental analysis missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, isn't logically developed 4 __/4
Total 10 __/10