BISC110/S10:Schedule
BISC110 Spring 2010 Lab Calendar
| Monday | Tuesday | Wednesday | Thursday | Friday | |
|---|---|---|---|---|---|
| January 25 |
January 26 Lab 1 |
January 27 Lab 1 |
January 28 Lab 1 |
January 29 Lab 1 |
|
| February 1 Lab 1 |
February 2 Lab 2 |
February 3 Lab 2 |
February 4 Lab 2 |
February 5 Lab 2 |
|
| February 8 Lab 2 |
February 9 Lab 3 |
February 10 Lab 3 |
February 11 Lab 3 |
February 12 Lab 3 |
|
| February 15 Presidents' Day No Lab |
February 16 Monday Schedule Lab 3 |
February 17 No Lab |
February 18 No Lab |
February 19 No Lab |
|
| February 22 Lab 4 |
February 23 Lab 4 |
February 24 Lab 4 |
February 25 Lab 4 |
February 26 Lab 4 |
|
| March 1 Lab 5 |
March 2 Lab 5 |
March 3 Lab 5 |
March 4 Lab 5 |
March 5 Lab 5 |
|
| March 8 Lab 6 |
March 9 Lab 6 |
March 10 Lab 6 |
March 11 Lab 6 |
March 12 Lab 6 |
|
| March 15 Lab 7 |
March 16 Lab 7 |
March 17 Lab 7 |
March 18 Lab 7 |
March 19 Lab 7 |
|
| March 22 Spring Break |
March 23 Spring Break |
March 24 Spring Break |
March 25 Spring Break |
March 26 Spring Break |
|
| March 29 Lab 8 |
March 30 Lab 8 |
March 31 Lab 8 |
April 1 Lab 8 |
April 2 Lab 8 |
|
| April 5 Lab 9 |
April 6 Lab 9 |
April 7 Lab 9 |
April 8 Lab 9 |
April 9 Lab 9 |
|
| April 12 Lab 10 |
April 13 Lab 10 |
April 14 Lab 10 |
April 15 Lab 10 |
April 16 Lab 10 |
|
| April 19 Patriots' Day No Lab |
April 20 Lab 11 |
April 21 Lab 11 |
April 22 Monday Schedule Lab 11 |
April 23 Lab 11 |
|
| April 26 No Lab |
April 27 No Lab |
April 28 Rhulman No Lab |
April 29 Lab 11 |
April 30 Lab 12 |
|
| May 3 Lab 12 |
May 4 Lab 12 |
May 5 Lab 12 |
May 6 Last Day of Classes Lab 12 |
Schedule of Experiments
| Section | Lab # | Activities |
|---|---|---|
| 1 | 1-5 | Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing |
| 2 | 6-7 | Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis |
| 3 | 8-10 | Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems |
| 4 | 11 | Gene Regulation/Enzymology: Invertase enzyme assay |
| Lab Practical | 12 | Hands-on skills assessment: |
Assignments
BISC110 Assignments/ Weekly Lab Planner
| Series | Lab # | LAB DATES__ | LAB DESCRIPTION________ | Assignment/Notes | ||
| 1 | 1 | Tues. Jan.26- Mon. Feb 1 |
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis |
Homework: Familiarize yourself with the wiki and particularly the information in the BISC110:Resources section of the Lab wiki; Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_ProblemsS10.doc; Read about the process of endocytosis in your textbook and familiarize yourself with phagocytosis in Tetrahymena from the following reference articles: Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097; | ||
| 1 | 2 | Tues. Feb.2- Mon. Feb.8 |
Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. | Homework: Study dilution making for Lab 3; Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data. | ||
| 1 | 3 | Tues. Feb. 9- Tues. Feb. 16 |
Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis | Homework: There are animated tutorials on dilution making available at the following link: Dilution Help Tutorials; Finalize your experimental design for Lab 4 and have it approved by your lab instructor; Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends; Work the dilution problems found at Media: Lab_3_S10_Dilution_Problems.doc and hand them in in Lab 4. | ||
| 1 | 4 | Mon. Feb. 22- Fri. Feb. 26 |
Student Designed Experiments: Potential Inhibitors of Vacuole Formation; | Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. Read assigned journal article for science writing discussion [Link to Wellesley College Library] | ||
| 2 | 5 | Mon. Mar.1- Fri. Mar. 5 |
Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop; | Homework: Partial lab report: Title Page, Results, Discussion and References sections. Guidelines at:BISC110/S10:Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources. Have Lab notebooks ready to be turned in for inspection after Lab 6.
| ||
| 2 | 6 | Mon. Mar. 8- Fri. Mar.12 |
Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; | Homework: Read the following background information on restriction enzymes found at:(http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf)
and on gel electrophoresis at: | ||
| 2 | 7 | Mon. Mar.15- Fri. Mar. 19 |
Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Determine PTC Taster Genotype; Scientific Writing Workshop | Homework:Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. Due for all students Friday, April 2.For help, see the Resources section of this wiki. | ||
| 3 | 8 | Mon. Mar. 29- Fri. Apr. 2 |
Photosynthesis 1: Photosynthetic pigments | Homework: Prepare a full results section for the photosynthetic pigment identification experiment; In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9); | ||
| 3 | 9 | Mon. April 5- Fri. Apr. 9 |
Photosynthesis 2: Hill Reaction | Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction; Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | ||
| 3 | 10 | Mon. Apr. 12- Fri. Apr. 16 |
Photosynthesis 3: Investigation of variables affecting the Hill Reaction | Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments with all or some of the treatments on the same graph.(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis); A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis. Compare your results to other investigations published in the primary literature. Refer to the " Guidelines for science writing" information found in the Resources section of the wiki. | ||
| 4 | 11 | Tues. Apr. 20- Thurs. Apr. 29 |
Enzymology | Homework: Prepare for a Lab Practical Skills Assessment in lab 12. Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions_110.doc
| ||
| Skills Assessment | 12 | Fri Apr. 30- Thurs. May 6 |
Lab Practical; Turn in Lab Notebook |
End of lab | ||
Lab Graded Assignments
| Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
|---|---|---|---|
| 1 | 2 | Practice Problems | 5 |
| 2 | 3 | Fig. Design (ungraded) | 0 |
| 3 | 4 | Dilution Problems (5); Fig.& Results (10) |
15 |
| 4 | 5 | Oral presentation: Results, original figure & legend; Journal article discussion |
10 |
| 5 | 6 | Lab notebook check (ungraded); Partial Scientific Paper(25) |
25 |
| 6 | 7 | SNP Taster Questions | 5 |
| 7 | Due for all students Fri. 4/2 |
Taster Scientific Paper (all but M&M) | 35 |
| 8 | 9 | Photosyn. Pigments Results | 10 |
| 9 | 10 | M&M (5pt); Finalize Self-Design Experiment Hill Rxn.(ungraded) |
5 |
| 10 | 11 | Full Scientific Paper Hill Rxn. | 35 |
| 11 | 12 | Lab Notebook Check (10) Concept Questions Enzymology/Gene Reg. (5) |
15 |
| 12 | 12 | Lab Practical:Hands-on skills assessment |
35 |
| Other | - | Discretionary Points Preparation & Participation |
5 |
