BCH4160/2011:Notebook/Laura Lee/2011/09/20

Preparation of Lipid Films

The purpose of this protocol was to prepare lipid films for use in future experiments.

Materials

Materials below provided by Dr. J. Cannon.

• CHCl3
• MeOH
• Lipid solution
• Vial

Procedure

1. Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH.
2. Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total).
3. Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total).
4. Dry the vials using a roto-evaporator.
5. Place vial in a desicator overnight to thoroughly dry.
6. Store vials in freeze for future use.