BCH4160/2011:Notebook/Laura Lee/2011/08/30

Buffer

The objective of this protocol was to make buffers of various pHs.

Materials

• Distilled water
• 0.2M Dibasic sodium phosphate
• 0.1M Citric acid

Procedure

• Note: need to test pH after making to guarantee correct pH.

Sodium Phosphate Buffers (pKa: 6.87)

Theoretical pH	0.1 Citric Acid (mL)	0.2M Sodium Phosphate (mL)
4.0	61.45	38.55
4.2	58.60	41.40
5.2	46.40	53.60
6.0	36.85	68.15
6.7	27.25	72.75
7.2	13.05	86.95
7.4	9.15	90.85

Contact

This experiment was done with High Point University student Jigesh Patel.