From OpenWetWare
(Redirected from BBb)
Jump to navigationJump to search

You have reached the home for development of the BglBrick standard for DNA assembly (formerly known as BBb). You are welcome to ask questions, make suggestions, and propose sub-standards in the spaces below.

At its core, the BglBrick standard simply means an idempotent standard assembly scheme using BamHI and BglII. The primary formal definition of the standard is:

The BglBrick Format

The BglBrick standard was developed by several researchers at UC Berkeley and is based on idempotent assembly with BamHI and BglII restriction enzymes. In a nutshell, most plasmids look like this:


and BglBrick scars are "GGATCT" encoding gly-ser when translated in frame. Note, however, that BglBrick is intended as a minimal physical assembly standard, and only those features needed for interconversion of BglBrick plasmids are formally defined. Therefore, "atg" and "taa" spacers are not core definitions of the standard.

The current method for expressing BglBrick parts in the Registry is to make them all basic parts with the sequence in between BglII and BamHI. So, all parts, basic, composite, and intermediate, are expressed as Basic Parts.

Formal Definition:

  • A BglBrick part is a DNA sequence flanked on the 5' end by "GATCT" and on the 3' end by "G" lacking BglII, BamHI, EcoRI, and XhoI restriction sites
  • A BglBrick vector is a DNA sequence flanked on its 5' end by "GATCC" and on its 3' end by "A"
  • A BglBrick entry vector has a unique EcoRI site, no BamHI or BglII restriction sites, and at most one XhoI site 5' to the EcoRI site
  • A BglBrick plasmid is represented as <vector_name>-<part_name> and has the sequence obtained by concatenating the vector and part sequences
  • Further definition constraints are "sub-standards" of the BglBrick format



Proposals for Sub-Standards

Chris Anderson's slides for the original BBb proposal. Media:potter-standards.ppt