Ampicilin Plates

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1. Weigh out 25g of LB Broth powder

2. Transfer to a large beaker and add 1000mL of DH2O; mix well

3. Split solution in half and put into two separate bottles

4. Add 7.5g of Agar to one of the bottles and mix well (note: don't forget to label bottles)

5. Autoclave both bottles (Setting: liquid-45)

6. Bring bottle with agar to a luke warm temperature

  • swirl it occasionally so it does not solidify on the bottom

7. Thaw and add ampilcilin to the mixture at 1:1000

8. Pour a thin layer of agar into each petri dish

  • mark petri dishes so that we know it is ampicilin

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