Amine coupling method in SPR

protein	conditions	immobilization level, RU	citation
HSA (Human Serum Albumin)	(1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min (2) HSA, 15 μg/mL same buffer 7 min (3) 1M ethanolamine, pH 8.5, 7 min (block unreacted active esters) (4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein)	9,500-11,000	[1]

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