Amine coupling method in SPR

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protein conditions immobilization level, RU citation
HSA (Human Serum Albumin) (1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min

(2) HSA, 15 μg/mL same buffer 7 min
(3) 1M ethanolamine, pH 8.5, 7 min (block unreacted active esters)
(4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein)

9,500-11,000 [1]
  1. Frostell-Karlsson A, Remaeus A, Roos H, Andersson K, Borg P, Hämäläinen M, and Karlsson R. Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels. J Med Chem. 2000 May 18;43(10):1986-92. DOI:10.1021/jm991174y | PubMed ID:10821711 | HubMed [karlsson1]