Alik:Protocols (Media)

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3T3 media

450 mL Regular DMEM
50 mL (1 tube) FBS
5 mL Pen-Strep

ES media (mouse)

~425 mL (416.5 mL to be exact) Knockout DMEM
75 mL (2 tubes) ES-FBS
50uL LIF
1 mL beta-mercaptoethanol
5 mL non-essential amino acids
2.5 ml (1 tube) L-glutamine
5 mL Pen-Strep

EB media (mouse)

(Same as ES media, only without Lif)

~425 mL (416.5 mL to be exact) Knockout DMEM
75 mL (2 tubes) ES-FBS
1 mL beta-mercaptoethanol
5 mL non-essential amino acids
2.5 ml (1 tube) L-glutamine
5 mL Pen-Strep