AU Biomaterials Design Lab:Protocols/Expression Culture Media

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The expression culture will be able to support a large number of bacteria as they express our protein of interest. We will usually make 4 flasks-worth of expression culture.


  • This needs to be done the day before protein expression
  1. Prepare LB in a 2.8L Fernbach flask
    1. 25g of LB
    2. 1L of water
  2. Cover the flask with foil
  3. Autoclave (You can only fit two flasks into the autoclave at one time so you need to plan accordingly and perform two runs in the autoclave)
  4. Allow the flask to cool
  5. Add 1mL of 1000X Antibiotic