AG Daniel:cell lines
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AG Daniel
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wild type
| name | cell type | deletions | abberations | S level | medium | storage |
|---|---|---|---|---|---|---|
| DOHH-2 | human follicular lymphoma (b-cell) | t(14;18) → Bcl-2 | S2 (EBV) | RPMI + 10% FCS | N2 Tank 1 Ecke D2 | |
| DU-145 | human prostate carcinoma | Bax, p53 | S1 | RPMI + 10% FCS | ||
| GRANTA-519 | human mantle cell lymphoma (b-cell) | t(11;14) → Cyclin D1 | S2 (EBV) | RPMI/DMEM + 10% FCS | N2 Tank 1 Ecke D1 | |
| HCT116 | human colon carcinoma | S1 | DMEM/McCoys + 10% FCS | |||
| JEKO-1 | human mantle cell lymphoma (b-cell) | Bim, p53 | t(11;14) → Cyclin D1 | S1 | RPMI + 10-20% FCS | N2 Tank 1 Ecke D1 |
| JVM-2 | human mantle cell lymphoma (b-cell) | t(11;14) → Cyclin D1 | S1 | RPMI + 10% FCS | -80°C AnjaM | |
| KARPAS-422 | human follicular lymphoma (b-cell) | t(14;18) → Bcl-2 | S2 (EBV) | RPMI + 10% FCS | N2 Tank 1 Ecke D2 | |
| MINO | human mantle cell lymphoma (b-cell) | Bim | t(11;14) → Cyclin D1 | S1 | RPMI + 10% FCS | N2 Tank 1 Ecke D1 |
| NCEB-1 | human mantle cell lymphoma (b-cell) | t(11;14) → Cyclin D1 | S2 (EBV) | RPMI + 10% FCS | N2 Tank 1 Ecke D1 | |
| REC-1 | human mantle cell lymphoma (b-cell) | t(11;14) → Cyclin D1 | S1 | RPMI + 10% FCS | N2 Tank 1 Ecke D1 | |
| RL | human follicular lymphoma (b-cell) | t(14;18) → Bcl-2 | S1 | RPMI + 10% FCS | N2 Tank 1 Ecke D3 | |
| SC-1 | human follicular lymphoma (b-cell) | t(14;18) → Bcl-2 | S1 | RPMI + 10% FCS | N2 Tank 1 Ecke D2 | |
| WSU-NHL | human follicular lymphoma (b-cell) | t(14;18) → Bcl-2 | S1 | RPMI + 10% FCS | N2 Tank 1 Ecke D2 | |
| SW48 | ||||||
| SW-480 | human colon adenocarcinoma | |||||
| U2OS | human osteosarcoma cell line | p16 | ||||
| SAOS-2 | human osteogenic sarcoma | |||||
| MEF | mouse embryonic fibroblast | S1 | ||||
| LOVO | human colon adenocarcinoma | S1 |
transfectants
| name | change | vector | resistance | S level | storage | comment |
|---|---|---|---|---|---|---|
| HCT116 p53-/- | p53 knockout | targeted deletion of p53 | neomycin and hygromycin | S1 | The p53 knockout variant 379.2 of HCT-116 cells was kindly provided by Dr. Bert Vogelstein (John Hopkins University, Baltimore, MD). Literature | |
| HCT116 p21-/- | p21 knockout | S1 | HCT116 p21 knockout was kindly provided by Drs. T. Waldman and B. Vogelstein (John Hopkins University, Baltimore, MD). | |||
| HCT116 Puma-/- | Puma knockout | S1 | ||||
| HCT116 wt + pIRESneo3 | pIRESneo3 | neomycin | S1 | |||
| HCT116 wt + ΔNp53 | dominant negative N-terminally truncated p53 | pIRESneo3 | neomycin | S1 | ||
| HCT116 wt + CHOP | pIRESneo3 | neomycin | S1 | |||
| HCT116 wt + CHOP Δ95 | pIRESneo3 | neomycin | S1 | |||
| HCT116 wt + CHOP Δ130 | pIRESneo3 | neomycin | S1 | |||
| DU145 HyTk2 | HyTk? | hygromycin | S1 | |||
| DU145 Bax3 | Bax Klon #3 in HyTk | hygromycin | S1 |
