AG Daniel:cell lines

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AG Daniel

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Forschungshaus Charité
Lindenberger Weg 80
13125 Berlin

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wild type

name cell type deletions abberations S level medium storage
DOHH-2 human follicular lymphoma (b-cell) t(14;18) → Bcl-2 S2 (EBV) RPMI + 10% FCS N2 Tank 1 Ecke D2
DU-145 human prostate carcinoma Bax, p53 S1 RPMI + 10% FCS
GRANTA-519 human mantle cell lymphoma (b-cell) t(11;14) → Cyclin D1 S2 (EBV) RPMI/DMEM + 10% FCS N2 Tank 1 Ecke D1
HCT116 human colon carcinoma S1 DMEM/McCoys + 10% FCS
JEKO-1 human mantle cell lymphoma (b-cell) Bim, p53 t(11;14) → Cyclin D1 S1 RPMI + 10-20% FCS N2 Tank 1 Ecke D1
JVM-2 human mantle cell lymphoma (b-cell) t(11;14) → Cyclin D1 S1 RPMI + 10% FCS -80°C AnjaM
KARPAS-422 human follicular lymphoma (b-cell) t(14;18) → Bcl-2 S2 (EBV) RPMI + 10% FCS N2 Tank 1 Ecke D2
MINO human mantle cell lymphoma (b-cell) Bim t(11;14) → Cyclin D1 S1 RPMI + 10% FCS N2 Tank 1 Ecke D1
NCEB-1 human mantle cell lymphoma (b-cell) t(11;14) → Cyclin D1 S2 (EBV) RPMI + 10% FCS N2 Tank 1 Ecke D1
REC-1 human mantle cell lymphoma (b-cell) t(11;14) → Cyclin D1 S1 RPMI + 10% FCS N2 Tank 1 Ecke D1
RL human follicular lymphoma (b-cell) t(14;18) → Bcl-2 S1 RPMI + 10% FCS N2 Tank 1 Ecke D3
SC-1 human follicular lymphoma (b-cell) t(14;18) → Bcl-2 S1 RPMI + 10% FCS N2 Tank 1 Ecke D2
WSU-NHL human follicular lymphoma (b-cell) t(14;18) → Bcl-2 S1 RPMI + 10% FCS N2 Tank 1 Ecke D2
SW48
SW-480 human colon adenocarcinoma
U2OS human osteosarcoma cell line p16
SAOS-2 human osteogenic sarcoma
MEF mouse embryonic fibroblast S1
LOVO human colon adenocarcinoma S1

transfectants

name change vector resistance S level storage comment
HCT116 p53-/- p53 knockout targeted deletion of p53 neomycin and hygromycin S1 The p53 knockout variant 379.2 of HCT-116 cells was kindly provided by Dr. Bert Vogelstein (John Hopkins University, Baltimore, MD). Literature
HCT116 p21-/- p21 knockout S1 HCT116 p21 knockout was kindly provided by Drs. T. Waldman and B. Vogelstein (John Hopkins University, Baltimore, MD).
HCT116 Puma-/- Puma knockout S1
HCT116 wt + pIRESneo3 pIRESneo3 neomycin S1
HCT116 wt + ΔNp53 dominant negative N-terminally truncated p53 pIRESneo3 neomycin S1
HCT116 wt + CHOP pIRESneo3 neomycin S1
HCT116 wt + CHOP Δ95 pIRESneo3 neomycin S1
HCT116 wt + CHOP Δ130 pIRESneo3 neomycin S1
DU145 HyTk2 HyTk? hygromycin S1
DU145 Bax3 Bax Klon #3 in HyTk hygromycin S1