Yu:SOC: Difference between revisions
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m (Touchup) |
m (Fixed) |
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Line 18: | Line 18: | ||
|2.5mM KCl | |2.5mM KCl | ||
|- | |- | ||
|6. Pipette | |6. Pipette 1mL of 1M MgCl<sub>2</sub> into the mixture | ||
|10mM MgCl<sub>2</sub> | |10mM MgCl<sub>2</sub> | ||
|- | |- | ||
|7. Weigh 0. | |7. Weigh 0.12g anhydrous MgSO<sub>4</sub> and add it to the mixture | ||
|10mM MgSO<sub>4</sub> | |10mM MgSO<sub>4</sub> | ||
|- | |- |
Latest revision as of 12:16, 29 December 2011
1. Measure 50mL millipore water into a 200mL beaker. | |
2. Weigh 2g Tryptone and add it to the mixture | 2% (w/v) Tryptone |
5. Weigh 0.5g yeast extract and add it to the mixture | 0.5% (w/v) Yeast Extract |
4. Pipette 200μL of 5M NaCl into the mixture | 10mM NaCl |
5. Pipette 83μL of 3M KCl into the mixture | 2.5mM KCl |
6. Pipette 1mL of 1M MgCl2 into the mixture | 10mM MgCl2 |
7. Weigh 0.12g anhydrous MgSO4 and add it to the mixture | 10mM MgSO4 |
8. Weigh 0.36g dextrose and add it to the mixture | 20mM dextrose |
9. Bring to 100mL with millipore water |