Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2012/11/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
 
(6 intermediate revisions by the same user not shown)
Line 9: Line 9:
  
  
==CNTI Acos5==
+
==CNHY MP1a/b Optimization==
 +
* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
 +
* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
 +
* CNHY MP1a: decrease Acos5
 +
* CNHY MP1b: increase Ai5057, Ai5062
  
  
===PCR===
+
===Gel===
* [[Image:20120110_PCRa.png|800 px]]
+
* too lazy to do confirmation gel
 +
 
 +
 
 +
===Fragment Analysis Submission===
  
  
===Gel===
+
* UAGC Submission# YFPQ
 +
:: [[Image:20121115_Frag.png|900 px]]
  
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
 +
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184817 801124184817]
 +
:: [[Image:20121115_FedEx.png|700 px]]
  
[[Image:20120110_Gela.png|800 px]]
 
  
* mispriming everywhere - primer pair not usable for CNHY
+
* FedEx picked up 3:01pm, 15 November
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
* FedEx delivered 9:23am, 16 November
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
* UAGC received 12:08pm, 16 November
 +
* UAGC completed 11:31am, 19 November
  
  

Latest revision as of 15:18, 27 November 2012

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


CNHY MP1a/b Optimization

  • optimize for marker peak height - vary each primer (based on results from 23 July 2012)
  • CNHY MP1a: increase Ai5013, Ai5043, Ai5071
  • CNHY MP1a: decrease Acos5
  • CNHY MP1b: increase Ai5057, Ai5062


Gel

  • too lazy to do confirmation gel


Fragment Analysis Submission

  • UAGC Submission# YFPQ
20121115 Frag.png


20121115 FedEx.png


  • FedEx picked up 3:01pm, 15 November
  • FedEx delivered 9:23am, 16 November
  • UAGC received 12:08pm, 16 November
  • UAGC completed 11:31am, 19 November