User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/16: Difference between revisions
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==BSA and HAuCl<sub>4</sub> Solutions== | |||
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
**MW = 393.83 g/mol | |||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
***actual amount used: 10.01mg | |||
***therefore, I need 11.0048mL H<sub>2</sub>O | |||
*BSA solution | |||
**MW = 66776g | |||
**in 20mL 15μM solution, I need 20.032mg BSA | |||
***actual amount used: 20.120mg BSA | |||
***therefore, I need 20.0879mL of H<sub>2</sub>O | |||
==Gold:BSA solutions, 60/90/120/150 Ratios== | ==Gold:BSA solutions, 60/90/120/150 Ratios== | ||
*Make stock solutions of 2.5mM Au and 15μM BSA | *Make stock solutions of 2.5mM Au and 15μM BSA | ||
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**new BSA: 0.00167mM | **new BSA: 0.00167mM | ||
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O | **solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O | ||
*All | *All 4 solutions put into the 80°C oven at | ||
==Autoclaving: BSA + Au:BSA solutions== | ==Autoclaving: BSA + Au:BSA solutions== |
Revision as of 06:35, 16 July 2013
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BSA and HAuCl4 Solutions
Gold:BSA solutions, 60/90/120/150 Ratios
Autoclaving: BSA + Au:BSA solutions
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