Difference between revisions of "User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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***BSA solution = 10.57mL
 
***BSA solution = 10.57mL
  
*1mL was siphoned from each solution and added to 8mL of purified water.
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*1mL was siphoned from each stock solution and added to 8mL of purified water.
  
 
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours.
 
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours.
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==Gel==
 
==Gel==
 
*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
 
*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
**This solution was heated and stirred for approximately 50 minutes until a homogeneous gel remained
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**This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
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*1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H<sub>2</sub>O solution
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**After stirring the new solution, it was placed in the oven
  
  

Latest revision as of 22:42, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
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Nanoparticle Fiber

  • Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
    • Concentrations:
      • Au = .25 mM
      • BSA = 1.785 μM
    • Amounts:
      • Au = .4924 mg
      • BSA = 1.19 mg
  • Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
    • Theoretical Amounts:
      • Au = 4.924 mg
      • BSA = 11.9 mg
    • Actual amounts used:
      • Au = 5.3 mg
      • BSA = 12.6 mg
    • Adjusted mL based on extra amounts:
      • Au solution = 10.76mL
      • BSA solution = 10.57mL
  • 1mL was siphoned from each stock solution and added to 8mL of purified water.
  • The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.

Gel

  • 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
    • This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
  • 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
    • After stirring the new solution, it was placed in the oven